Journal: Genetics and molecular research : GMR

Article Title: Active vitamin D3, 1,25-(OH)2D3, protects against macrovasculopathy in a rat model of type 2 diabetes mellitus.

doi: 10.4238/gmr.15028113

Figure Lengend Snippet: Figure 5. CD54 expression in the thoracic aorta of rats in (A) control group, (B) T2DM (type 2 diabetes mellitus) group, and (C) treatment group. (Immunohistological staining, 400X).

Article Snippet: Streptozotocin (Sigma-Aldrich, USA), calcitriol (Roche, Shanghai, China), monoclonal antibodies against TNF-α, ET, eNOS, CD54, and CD106 (Boster, Wuhan, China), PV-9000 Polymer Detection System for Immuno-Histological Staining, DAB kit (ZSGB-Bio, Beijing, China), and full-automatic biochemical analyzer (ABX, France) were used in this experiment.

Techniques: Expressing, Control, Staining

Journal: Infection and Immunity

Article Title: Rickettsia rickettsii inactivated whole cell antigen vaccine protects against Rocky Mountain spotted fever independent of the adjuvant used

doi: 10.1128/iai.00412-25

Figure Lengend Snippet: WCAV limits vasculitis. Concentrations of released ICAM-1 and VEGF were evaluated by enzyme-linked immunosorbent assay. Secretion of ICAM-1 remains low in vaccinated animals (red lines), while significantly higher in unvaccinated controls (black lines) from day 3 to day 9 post-infection challenge. The vaccinated and control groups were compared by two-way ANOVA test followed by multiplicity-adjusted post hoc comparisons (Tukey), and P -value < 0.05 was considered statistically significant (* P ≤ 0.05 and ** P ≤ 0.01).

Article Snippet: Plasma levels of ICAM-1 were analyzed for different time points by using commercial ELISA kits (Cusabio Dog intercellular adhesion molecule 1, ICAM-1/CD54 ELISA Kit).

Techniques: Enzyme-linked Immunosorbent Assay, Infection, Control